Table 2.

Sequence of primers used for RT-PCR analysis

	Gene Name	Gene ID No.	Sequence
β-Actin	ACTB	NM_001101.3	Forward: 5′-CAC TCT TCC AGC CTT CCT TC-3′
			Reverse: 5′-GGT GTA ACG CAA CTA AGT CAT AG-3′
Cyclin D1	CCND1	NM_053056.2	Forward: 5′-CAT CTA CAC CGA CAA CTC CAT C-3′
			Reverse: 5′-TCT GGC ATT TTG GAG AGG AGG-3′
β-Catenin	CTNNB1	NM_001098209.1	Forward: 5′-GTT CAG TTG CTT GTT CGT GC-3′
			Reverse: 5′-GTT GTG AAC ATC CCG AGC TAG-3′
Axin2	AXIN2	NM_004655.3	Forward: 5′-CCA CTG GCC GAT TCT TCC TT-3′
			Reverse: 5′-TAC CGG AGG ATG CTG AAG GC-3′
Dickkopf-1	DKK1	NM_012242.2	Forward: 5′-AGC GTT GTT ACT GTG GAG AAG-3′
			Reverse: 5′-GTG TGA AGC CTA GAA GAA TTA CTG-3′
p21	CDKN1A	NM_000389	Forward: 5′-GGA AGA CCA TGT GGA CCT GT-3′
			Reverse: 5′-GGC GTT TGG AGT GGT AGA AA-3′
Ki-67	MKI67	NM_001145966	Forward: 5′-ATT ATC GTT CCT TCA GGT ATG-3′
			Reverse: 5′-TCA TCA GGG TCA GAA GAG AA-3′
p16	CDKN2A, p16Ink4A	NM_000077	Forward: 5′-CAA CGC ACC GAA TAG TTA CG-3′
			Reverse: 5′-CAG CTC CTC AGC CAG GTC-3′