Fig. 3.
Effect of chronic alcohol exposure of pancreatic acinar 266-6 cells on carrier-mediated [3H]-thiamin uptake (A) and level of expression of THTR-1 and THTR-2 proteins (B) and mRNA (C). Cells were exposed chronically to alcohol (50 mM, 96 h), and carrier-mediated thiamin uptake was determined. Level of protein expression was determined by Western blotting. Levels of mRNA were determined by quantitative PCR. Data represent the means ± SE of at least 3 separate uptake determinations. *P < 0.01.