Fig. 7.

Effect of chronic exposure of pancreatic acinar 266-6 cells to alcohol on the level of expression of SP1 protein (A) and mRNA (B). Western blotting was performed on whole cell proteins (60 μg) isolated from alcohol-exposed and control 266-6 cells. Blot was incubated with rabbit polyclonal SP1 antibody. Q-PCR was performed using mice SP1 gene-specific primers and cDNA prepared from alcohol-exposed as well as control 266-6 cells mRNA. Data are means ± SE of at least 3 independent experiments and were normalized relative to ARPO and calculated by the relative relationship method. *P < 0.01, **P < 0.05.