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. 2014 Feb 5;306(7):F764–F772. doi: 10.1152/ajprenal.00426.2013

Fig. 1.

Fig. 1.

Cre-mediated excision of the conditionally floxed Dicer allele via the Pax3CreTg allele results in loss of microRNA (miRNA) expression in the metanephric mesenchyme at embryonic day 11.5 (E11.5). A–D: locked nucleic acid (LNA) in situ hybridization demonstrates loss of mmu-miR-10a and mmu-miR-17-5p expression in the metanephric mesenchyme of Pax3CreTg, Dicerflx/flx kidneys (B and D) compared with controls (A and C). Black dashed lines, metanephric mesenchyme; yellow dashed lines, ureteric bud. The magnification is ×20. E: quantitative real-time PCR performed on total RNA isolated from E11.5 kidneys confirms decreased expression of mmu-miR-10a and mmu-miR-17-5p (*P < 0.05, paired t-test). Relative expression of mmu-miR-10a and mmu-miR-17-5p denotes the fold-change in ΔCT in mutants normalized to controls. Bars represent SE.