Fig. 5.
β-Cell expression of a hyperactive GCK potentiates glucose-induced β-cell differentiation. A: schematic representation of the Tg(−1.2ins:GCKV91L; LR) transgene used to express a hyperactive human GCK mutant (GCKV91L) in β-cells. B: RT-PCR analysis of nontransgenic wild-type larvae or Tg(−1.2ins:GCKV91L; LR) transgenic larvae with (RT+) or without (RT−) reverse transcriptase confirmed expression of GCKV91L. C: the number of β-cells was significantly increased in Tg(−1.2ins:GCKV91L; LR);Tg(−1.2ins:H2BmCherry) transgenic larvae incubated in subthreshold 10 mM glucose compared with nontransgenic larvae. All values are means ± SE; n are shown inside of the bars. Groups labeled with different letters are significantly different from each other (P < 0.05).