Fig. 3.

Unloading-induced fiber-type shift is redox regulated in CON, HU, and HU-EUK rats (n = 8/group). A modified ATPase-staining technique revealed that significant shift in muscle soleus muscle fiber-type from fast to slow in the soleus as a result of HU. A: representative sections for both alkaline (pH = 10.4) and acidic (pH = 4.25) preincubations are presented. A reduction of slow-twitch fibers (Type I) was noted with HU (B) (P < 0.05), accompanied by an elevation in Type IIa (C), and Type IIb/d fibers (D). EUK-134 abrogated the unloading-induced fiber-type shift (A–D). ^a,bLetters that differ are significantly different from each other (P < 0.05).