Fig. 9.

Effects of CV4 6-OHDA administration on DVC corticotropin-releasing hormone (CRH)-immunoreactive nerve cell counts. Sections through the commissural and caudal DVC of SAL-injected vehicle- versus 6-OHDA-pretreated rats were processed for confocal microscopic analysis of CRH-ir. Top: data in the chart shown indicate that 6-OHDA did not alter mean bilateral counts of CRH-ir-positive neurons in either DVC region. Representative photomicrographs depict immunostaining for cytoplasmic CRH-ir alone (A and B) or merged CRH-ir and nuclear NAPI labeling (C and D) after vehicle (left-hand column) versus 6-OHDA (right-hand column) treatment.