Fig. 7.
Tube formation responses in VVEC and HPP-VVEC. A: tube formation was performed in angiogenesis slides (ibidi) as described in materials and methods. Growth-arrested VVEC and HPP-CFC (17,000/well) were seeded on polymerized Growth Factor Reduced Matrigel and incubated for 6 h with or without ADP (100 μM). B: evaluation of tube formation responses in VVEC and HPP-CFC were carried out using the Image-Pro Premier 9.0 software. VVEC-Co and VVEC-Hx (n = 3), HPP-CFC-Co (n = 3) and HPP-CFC-Hx (n = 6), each in triplicate. *P < 0.05 and ***P < 0.0001, control vs. hypoxic cells; #P < 0.05, ##P < 0.01, and ###P < 0.001, control vs. ADP-stimulated cells.