Fig. 4.

Effect of MJ33 (0.1 mg/kg) administered concurrently with LPS (1 mg/kg) on vascular endothelial cell adhesion molecule (VCAM) expression in the lung homogenate at 24 h after LPS. A: band at 110 kDa on Western blot indicates VCAM-1 expression for control (no LPS, no MJ33, lane 1), LPS (lane 2), and LPS + MJ33 (lane 3). The tubulin band, used as a loading control, was ∼55 kDa. The vertical dark line indicates the removal of several nonrelevant lanes. Anti-VCAM antibody was used at 1:1,000 and anti-tubulin antibody at 1:500. B: immunoblots were quantified by ImageJ software; values are expressed in arbitrary units for VCAM relative to the corresponding tubulin expression. Results are means ± SE for n = 3. *P < 0.01 compared with lane 2; #P < 0.05 compared with lane 1.