Table 2.
The sequences and positions of synthetic oligonucleotides used to generate mutants of Lef-1 promoter-luciferase constructs
| Binding Sitea | Lef-1 Promoter Position, bpb | Synthetic Oligonucleotide Sequencec |
|---|---|---|
| S1 | −2660/−2654 | 5′-GGATCCTTTGTAAAGTTTTAAAAATTGCTTCCTAGTAAcggaggATTCCCAAGAAGTAGTCGTTATTAGTAGGTGAAGTCATTAGTGC-3′ |
| S2 | −1956/−1950 | 5′-GTACATCCCGTGGTGAGAACAGAATGAAAGATATcggtggTAAAAAGCAATAATTAAAATCTAGTCTTCAGTTCCTTCTT-3′ |
| S3 | −780/−774 | 5′-GGAGTCCCCGCGCTTGCCGGCAAAAACTTTcggaggGGCAAAACTCCTCTTTCTCTTCCCCTCCTCCTCCTCGGGCC-3′ |
a
Three putative Sox binding sites within the human Lef-1 promoter were selected for mutation analysis.
b
Base pair position is relative to the translational ATG start site (+1) in exon 1 of the human Lef-1 gene.
c
The mutated sequences are shown in lowercase and bold.