FIG. 4.

AptamiR molecules interfere with endogenous miR21 function. Aptamer-triggered import of antimiR-21 into breast cancer cells was investigated employing a recombinant MCF-7 cell line that contains a miR-21-targeted site in the 3′-untranslated region of enhanced green fluorescent protein (EGFP). Oligonucleotides were directly added to the culture media at the indicated concentrations (a) or at 5μM in (b) and induced EGFP-fluorescence was analyzed 72 hours after incubation by flow cytometry (a) and fluorescence microscopy (b).