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. Author manuscript; available in PMC: 2014 Aug 24.
Published in final edited form as: Nat Commun. 2014;5:3349. doi: 10.1038/ncomms4349

Figure 6. Changes in holding current with DHA and antagonists.

Figure 6

(a) Whole-cell current evoked by 10 μM NMDA and 10 μM glycine (open bars) before and after exposure to 15 μM DHA (black bars). The glycine site antagonist ACEA 1021 (1 μM) was applied together with DHA during the periods indicated by the grey bars. After the final exposure to DHA the control external solution contained 0.1% BSA. (b) Plot of current evoked immediately after exposure to DHA (mean ± s.e.m.) as a fraction of control current before DHA for the 12 chimaeric constructs in Fig. 4 versus change in holding current with DHA exposure normalized to the current evoked by NMDA and glycine. Pearson product moment correlation coefficient = 0.949 (P < 0.0001). Sample size as given in Fig. 4e and 5a. (c) Percent block of DHA induced change in holding current (mean ± s.e.m.) for N1/K2(R)L614A + N2B/K2(R)L614A by 1 μM ACEA 1021 or 1 mM 5F-I2CA (20 cells) or by 50 μM APV or 30 μM CPP. Sample size is shown for each bar. (d) Percent change in baseline holding current (mean ± s.e.m.) without DHA exposure.