Fig. 2.

WT1(RP23-8C14)-Cre disrupts expression of GATA6 and GATA4 in the epicardial and sub-epicardial cells. The epicardial expression of GATA6 and GATA4 was visualized with immunofluorescence for GATA6 (A)–(D) and for GATA4 (Red, (E)–(H)). GATA6 (A) and (C) and GATA4 (E) and (G) were found to be expressed in epicardial and sub-epicardial cells of wild type hearts. In dcKO hearts, there is was an observable decrease in the epicardial expression of GATA6 (B) and (D) and GATA4 (F) and (H) when compared to the wild type (A), (C), (E) and (G). Hearts were co-stained against sarcomeric myosin (Green) to visualize the myocardium and DAPI (Blue) to visualize nuclei. Scale bars represent 50 µm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)