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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Dev Biol. 2013 Dec 28;386(1):204–215. doi: 10.1016/j.ydbio.2013.12.033

Fig. 5.

Fig. 5

dcKO hearts exhibit decreased SEECs and inner-myocardial endothelial cells. The presence of SEECs was observed by immunofluorescent labeling of endothelial cells with an anti-ERG antibody (Red, (A)–(D)).We determined the percentage of SEECs by counting the number of SEECs and dividing that value by the total number of epicardial and sub-epicardial cells. We observed a significant decrease in the percentage of sub-epicardial endothelial cells in the dcKO hearts (B) and (D) when compared to the wild type hearts (A) and (C). This decrease was found to be statistically significant (* = p < 0.005) at both E13.5 (E) and E14.5 (F). Additionally, we found decreases in the percentage of SEECs in WT1(RP23-8C14)-Cre+/− Gata4f/f Gata6f/+ (4f/f 6f/+) and WT1(RP23-8C14)-Cre+/− Gata4f/+ Gata6f/f hearts(4f/+ 6f/f, (E)–(F), * = p < 0.005). (G) dcKO hearts display a statistically significant (* = p < 0.05) decrease in the number of inner-myocardial endothelial cells at E13.5. Hearts were co-stained against sarcomeric myosin (MF20, Green) to visualize the myocardium and DAPI to label nuclei. All scale bars represent 50 µm. Error bars represent standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)