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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Dev Biol. 2013 Dec 28;386(1):204–215. doi: 10.1016/j.ydbio.2013.12.033

Fig. 6.

Fig. 6

WT1(RP23-8C14)-Cre expression is limited in SEECs. Embryos generated from mating WT1(RP23-8C14)-Cre mice with Rosa26R-eYFP reporter mice were stained with antibodies against eYFP (Green) and ERG (Red) to label endothelial cells. At E13.5 (A) and E14.5 (B) we observed eYFP negative SEECs along with a few eYFP positive, SEECs (arrows). The percentage of eYFP+ SEECs was found to be 16.89% at E13.5 and 19.58% at E14.5 (C). At E13.5, we found a statistically significant decrease in the percentage of SEECs in dcKO hearts when compared to wild type hearts (* = p < 0.005, (C)). We found no significant difference in the proliferation rates between eYFP+ and eYFP− SEECs at E12.5 (D). All scale bars represent 50 µm. Error bars represent standard deviation. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)