Fig. 5.

Replication of parental CPX 91/3, mutants and revertants in BS-C-1 cells and MEFs: (A) BS-C-1 cells were infected with 5 PFU/cell of parental 91/3 clone, ΔATI, ΔA26, ATIRev or A26Rev. At the indicated times, the cells were lysed and combined with the medium and virus titered. (B) BS-C-1 cells were infected with 0.05 PFU/cell of the indicated viruses and the cells and media were obtained at the indicated times and titered. (C, D) BS-C-1 cells were infected as in panel B but virus yields of cells alone and medium alone are shown in panels C and D, respectively. (E, F) MEFs were infected with 0.05 PFU/cell of the indicated viruses and virus yields of cells alone and medium alone are shown in panels E and F, respectively. Virus titers were obtained by plaque assay on BS-C-1 cells in duplicate from three separate infections (panels A, B, E, F) or two separate infections (panels C, D). Standard error bars (plus) are shown for panels A, B, E and F.