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. Author manuscript; available in PMC: 2014 Mar 22.
Published in final edited form as: Photochem Photobiol. 2013 Jul 8;89(5):1199–1207. doi: 10.1111/php.12104

Figure 2.

Figure 2

Ultrafiltered blackberry juice (UFT BBJ) does not protect normal human epidermal keratinocytes (NHEKs) against UVB-mediated decrease in cell viability and total cellular oxidation induced by UVB. NHEKs were treated with UFT BBJ for 2 h in the pretreatment and 24 h in the posttreatment, before or after exposure to UVB (25 mJ cm−2). Nonirradiated cells were treated with UFT BBJ for 24 h as controls. (a) Cells were harvested 24 h after UV irradiation, and percent cell viability was determined by MTT assay. (b) Phase contrast microscopy (10 ×) showing NHEKs treated with UFT BBJ before or after UVB irradiation (25 mJ cm−2). (c) Antioxidant activity was measured in NHEKs cell lysates by 2,2′-azino-di-[3-ethylbenzthiazoline sulfonate] oxidation assay as trolox equivalents. Data shown represent the mean ± SD of three independent experiments in which each treatment was repeated in three wells. *P ≥ 0.05 versus untreated control, #P ≥ 0.05 versus UVB control.