Figure 6.
Ultrafiltered blackberry juice (UFT BBJ) increased UVB-mediated apoptosis in normal human epidermal keratinocytes (NHEKs). NHEKs were pre- (2 h) and posttreated (24 h) with UFT blackberry juice before or after UVB irradiation (25 mJ cm−2) and harvested 24 h later. (a) UVB-mediated apoptosis (green) was detected by fluorescence microscopy using the annexin-V-FLUOS staining kit. Pictures shown are a representative of three independent experiments with similar results. (b) UVB-mediated poly(ADP-ribose) polymerase cleavage was determined by western blot. Equal loading was confirmed by reprobing the immunoblot for β-actin.