Limits of the accuracy of the parameter estimates as a function of the angular
offset of the starting point ψ0 of an object
with a circular trajectory. (a) Practical limit of R. Panel (b)
of xc. Panel (c) of yc.
Panel (d) of ω and (e) of
ψ0. (○) corresponds to a radius
of the circular trajectory of 350 nm while (*) corresponds to a radius
of 50 nm. The line style (– · –) corresponds to the
noise-free case while (—) refers to that of the fundamental limit. For
the object in all plots σ = 83 nm,
magnification M = 100, and the coordinates of
xc and yc are
(0, 0). The photon detection rate , period Tp
= 0.2 s, pixel size is 4.03 μm × 4.03
μm and the array size is 31 × 31
pixels.