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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Stroke. 2014 Jan 2;45(2):383–388. doi: 10.1161/STROKEAHA.113.003393

Figure 1.

Figure 1

Effect of knockdown or over-expression of ANRIL on regulation of CARD8 by quantitative RT–PCR (qRT–PCR) analysis. The mRNA samples were prepared from transfected HUVECs or HepG2 cells. ACTIN was used as a control for normalization. (A) CARD8 gene was significantly down-regulated about two fold by ANRIL knockdown in HepG2 cells. Expression of ANRIL was decreased by 5.7-fold and CARD8 expression was reduced by 2-fold. Transfection with pcDNA3.1-ANRIL increased the expression of ANRIL by 57-fold and CARD8 expression by 1.6-fold. Each experiment was preformed three times in triplicate. (B) CARD8 gene was significantly down-regulated about two fold by ANRIL knockdown in HUVECs. ***: P<0.001; **: P <0.01; *: P<0.05.