Skip to main content
. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Mol Cancer Res. 2013 Nov 18;12(3):370–380. doi: 10.1158/1541-7786.MCR-13-0281

Figure 2.

Figure 2

DDB2 inhibits the P1 promoter activity of bcl-2 gene. A, the schematic diagram of bcl-2 gene promoter structure. TSS: Translation start site. B, two luciferase reporters containing either P1+P2 or P2 only, empty or DDB2-expressing vector were transfected into CP70 cells. The relative promoter activity was calculated by normalizing to the empty vector (set as 100%). C, CP70 cells were transfected with DDB2-WT, DDB2-K244E, or empty vector for 48 h. The relative mRNA level was quantified by real-time PCR using GAPDH as endogenous control, and normalized to the empty vector (set as 100%). The upper panel represents DDB2 expression level in the transfected cells by immunoblotting using Tubulin as the loading control. D, CP70 cells were transfected with reporter construct P1+P2, DDB2-WT, DDB2-K244E, or empty vector for 48 h, the promoter activity was analyzed as in (B). n=3, bar: SD, *: p<0.05 by 2-sample t-test.