Fig. 3.
Effect of SphK inhibitors on the viability of PC12 and APP-transfected cells. Cell viability was determined spectrophotometrically using the MTT assay. (a) Concentration-dependent effect of SphK(s) inhibitors: DMS and SKI II on the viability of PC12 cells after 24 h incubation. (b) Effect of SphK(s) inhibitor: SKI II (10 μM) on APP-transfected cell viability. Data represent the mean value ± SEM for three separate experiments carried out in triplicate. ***p < 0.001 versus control (non-treated) PC12 cells; # p < 0.001 versus 20 µM DMS-treated PC12 cells; $ p < 0.001 versus 30 µM DMS-treated PC12 cells; & p < 0.001 versus 50 µM DMS-treated PC12 cells; ### p < 0.0001 versus non-treated PC12 cells; $$$ p < 0.0001 versus non-treated APPwt cells; &&& p < 0.0001 versus non-treated APPsw cells; *p < 0.05 versus SKI II-treated PC12 cells, using one-way ANOVA followed by Bonferroni’s Multiple Comparison Test