Fig. 6.
Effect of S1P on S1P1 antagonist-evoked decrease in cell viability. Cells were incubated with 1 µM S1P in the presence of 20 µM W123 for 24 h. Cell viability was spectrophotometrically determined using the MTT assay. Data represent the mean value ± SEM for three separate experiments carried out in triplicate. $ p < 0.05 versus non-treated APPwt cells; ## p < 0.01 versus non-treated APPsw cells; *p < 0.05 versus W123-treated PC12 cells; **p < 0.05 versus non-treated APPsw cells, using one-way ANOVA followed by post hoc Bonferroni’s Multiple Comparison Test