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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Ann Biomed Eng. 2013 Dec 3;42(2):243–259. doi: 10.1007/s10439-013-0952-x

Figure 7.

Figure 7

A comparison of CRISPR on- and off-target mutation rates at HBB and HBD, respectively (reproduced from 149). Cells were transfected with CRISPR plasmids with eight different HBB-directed guide strands: R-01 to R-08. After three days in culture, genomic DNA was amplified and the indel percentage determined using the T7E1 mutation detection assay.