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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Cell Stem Cell. 2013 Dec 5;13(6):676–690. doi: 10.1016/j.stem.2013.10.008

Figure 1. Thoc2 and Thoc5 expression correlates with the pluripotent state of ESCs.

Figure 1

(A) Expression of Thoc2 and Thoc5 during ESC differentiation. Thoc2, Thoc5, and Oct4 expression was determined by RT-qPCR at the indicated time points during ESC differentiation. Differentiation was induced by LIF-withdrawal, retinoic acid (RA) treatment, or embryoid body (EB) formation. Expression was normalized to day-0 by β-actin and plotted as mean ± SEM. (B) Oct4GiP reporter assay: the Oct4GiP reporter ESCs were transfected with non-targeting (NT), luciferase (Luc), Thoc2, or Thoc5 siRNAs, and the reporter activity was determined by fluorescence-activated cell sorting (FACS) analysis 96 hours after transfection. The percentage of differentiation was determined by the percentage of GFP-negative cells and plotted as mean ± SEM. (C) ESC morphology after Thoc2 and Thoc5 silencing. ESCs were transfected with the indicated siRNAs, and cellular morphology was examined 96 hours after transfection. See also Figure S1, S2.