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. Author manuscript; available in PMC: 2014 Dec 5.
Published in final edited form as: Cell Stem Cell. 2013 Dec 5;13(6):676–690. doi: 10.1016/j.stem.2013.10.008

Figure 2. Thoc2 or Thoc5 depletion results in loss of ESC self-renewal.

Figure 2

(A) Expression of the pluripotency genes after Thoc2 and Thoc5 silencing. Expression was determined by western blot 96 hours after siRNA transfections. GAPDH and RAN were used as loading controls. (B) Quantitation of the pluripotency protein expression based on western blot. Expression was normalized by GAPDH and compared to the NT-siRNA transfected cells, and plotted as mean ± SEM from four independent experiments. (C) Lineage marker expression after Thoc2 and Thoc5 silencing. Expression was determined by RT-qPCR 96 hours after siRNA transfection. Values are plotted as mean ± SEM from three independent experiments. (D) Venn diagram of genes differentially expressed 96 hours after Thoc2 or Thoc5 siRNA transfection based on microarray. (E) Pluripotency gene mRNA levels were determined by RT-qPCR 96 hours after siRNA transfection. Values are plotted as mean ± SEM from three independent experiments. See also Figure S3, Table S1, S2.