Figure 6. Percent inhibition of p75 receptor expression correlates directly with increase in TNF-mediated apoptosis and decrease in angiogenic gene expression in p75KD/LLCs.
Representative histograms of FACS analysis of propidium iodide (PI)-stained two p75KD/LLC (transfected with p75 shRNA plasmid combinations 1+2 and 1−4), where M1 gates represent sub-G0/G1 population of the cells with less than 2n DNA, presumably, apoptotic cells. B–E, Constitutive and TNF-mediated (80 ng/ml) expression (qRT-PCR analysis) for various angiogenic genes – B, VEGFA, C, HGF, C, PLGF and E, CXCR4 for the same two p75KD/LLCs 24 hrs post-TNF treatment.