Figure 2. Effect of chronic ethanol on hippocampal [³H]MPPF binding.

(A) Nissl stained section outlining the subregions and layers of the hippocampus. Representative control (B) and ethanol (C) autoradiograms showing high [³H]MPPF binding in CA1 and moderate to high binding in the dentate gyrus. Both ethanol and controls exhibited greater binding in posterior CA1 than intermediate levels in the stratum radiatum (E) and pyramidal cell layer (F). No differences were observed in the stratum lacunosum moleculare (D) or stratum oriens (G). Ethanol drinkers exhibited greater binding than controls in the molecular (H) and polymorphic (J) layers at both intermediate and posterior levels of the dentate gyrus. No differences were observed in the dentate gyrus granule cell layer (I). *p≤0.05; **p≤0.01; ^#p≤0.10. Abbreviations: CA1–3 – cornu ammonis 1–3; DG – dentate gyrus; gcl – granule cell layer; mol – molecular layer; pcl – pyramidal cell layer; pol – polymorphic layer; slm – stratum lacunosum moleculare; so – stratum oriens, sr – stratum radiatum.