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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Drug Alcohol Depend. 2014 Jan 15;136:135–142. doi: 10.1016/j.drugalcdep.2014.01.002

Figure 4. Effect of chronic ethanol on relative HTR1A and Yif1B mRNA expression.

Figure 4

Individual CA1 pyramidal neurons (A) and the dentate gyrus granule cell layer (C) were identified based on location, size and morphology in nissl stained sections. The discrete cell populations were laser capture microdissected (B, D) after which the isolated cells were visualized on the LCM cap (insets in B and D). Note that non-neuronal cell populations remain undissected (arrow heads in B) from cells of interest (arrows in B) using this technique. Chronic ethanol had no effect on HTR1A (E) or Yif1B (G) expression in the dentate gyrus. HTR1A expression was significantly lower in ethanol drinkers in intermediate than posterior CA1 pyramidal neurons (F). In contrast, Yif1B expression was significantly greater in posterior than intermediate CA1 pyramidal neurons of ethanol drinkers (H). *p≤0.05.