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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: Int J Obes (Lond). 2010 May 4;34(9):1355–1364. doi: 10.1038/ijo.2010.77

Figure 2.

Figure 2

Effect of Ad-36 on Cidec/FSP27, perilipin protein abundance and Cidec/FSP27 gene expression in human skeletal muscle (HSKM) cells. Cidec/FSP27 and perilipin protein abundance were measured in the muscle cells at day 6 after infection with doses of Ad-36 from 1.9 to 7.6 MOIs as indicated. Lysates (50 μg) were subjected to SDS–PAGE, and transferred to nitrocellulose membranes. Cidec/FSP27 and perilipin were detected with specific antibodies against Cidec/FSP27 and perilipin. Panel a shows Cidec/FSP27 and perilipin protein content in control, Ad-2 and Ad-36 infected muscle cells. Panel b reveals gene expression results of Cidec/FSP27 in control, Ad-2, Ad-36 infected muscle cells. The results were normalized by β-actin and represented as mean ± s.e.m. of three separated studies. ***P<0.001, Ad-36 vs control.