Abstract
A technique for obtaining intact mesophyll cell suspensions derived from higher plant leaves is described. A large number of taxonomically unrelated plants were found suitable for cell `extraction' including several plant species from monocotyledonous group.
The absorption spectra of leaf cells in suspension differed only slightly from that of Chlorella cells. The higher plant cells respired and photosynthesized in aqueous solutions very much like algal cell suspensions. Only osmotically intact cells photosynthesized maximally and their activity was stimulated by 2 to several fold by the addition of bicarbonate to the medium. The isolated cells when stored at low temperature with proper aeration maintained their initial activity for a minimum of 9 hr. Cells stored at the room temperature (27 ± 2°) lost their activity rather rapidly. The isolated cells were physiologically intact as tested by their metabolic response to diverse inhibiting chemicals and growth regulating substances.
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Selected References
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- Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ball E., Joshi P. C. Divisions in isolated cells of palisade parenchyma of Arachis hypogaea. Nature. 1965 Jul 10;207(993):213–214. doi: 10.1038/207213a0. [DOI] [PubMed] [Google Scholar]
- Jagendorf A. T., Evans M. The Hill Reaction of Red Kidney Bean Chloroplasts. Plant Physiol. 1957 Sep;32(5):435–440. doi: 10.1104/pp.32.5.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joshi P. C., Noggle G. R. Growth of Isolated Mesophyll Cells of Arachis hypogaea in Simple Defined Medium in vitro. Science. 1967 Dec 22;158(3808):1575–1577. doi: 10.1126/science.158.3808.1575. [DOI] [PubMed] [Google Scholar]
- Jyung W. H., Wittwer S. H., Bukovac M. J. Ion Uptake by Cells Enzymically Isolated from Green Tobacco Leaves. Plant Physiol. 1965 May;40(3):410–414. doi: 10.1104/pp.40.3.410. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- RACUSEN D. W., ARONOFF S. A homogeneous cell preparation from soybean leaves. Science. 1953 Sep 11;118(3063):302–304. doi: 10.1126/science.118.3063.302. [DOI] [PubMed] [Google Scholar]
- Torrey J. G., Reinert J. Suspension cultures of higher plant cells in synthetic media. Plant Physiol. 1961 Jul;36(4):483–491. doi: 10.1104/pp.36.4.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
- VOLKIN E., COHN W. E. Estimation of nucleic acids. Methods Biochem Anal. 1954;1:287–305. doi: 10.1002/9780470110171.ch11. [DOI] [PubMed] [Google Scholar]