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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: Biochem J. 2011 Mar 15;434(3):415–426. doi: 10.1042/BJ20101437

Figure 7. SPR-based equilibrium-binding analysis of the affinity for PtdIns(4,5)P2 and PtdIns(4,5)P2 for PBD1 and PBD2.

Figure 7

Either PBD1 (A–D) or PBD2 (E and F) were injected at 5 μl/min over immobilized PtdCho/PtdE/PtdIns(3,4)P2 vesicles (A and B) or PtdCho/PtdE/PtdIns(4,5)P2 vesicles (C–F) and the resulting changes in the RU are plotted as a function of time to yield the SPR binding sensorgrams, from which the Kd values were determined. Peptide concentrations ranged from 75 to 1900 nM.