Table 1.
Primer sequences used for quantitative PCR
| Primer | Sequence | Accession number | Amplicon length |
|---|---|---|---|
| DMD 5′ pig For (exon 9) | CCTCGGTTCAAGAGCTATGC | NM_001012408 | 128 |
| DMD 5′ pig Rev (exon 10) | TCCAACAATGAACTGCCAAA | ||
| DMD 5′ of SNP pig For (exon 37)a | AGCAAACTTGATGGCAAACC | NM_001012408 | 121 |
| DMD 5′ of SNP pig Rev (exon 38) | AATGGAGGCCTTTCCAGTCT | ||
| DMD across SNP pig For (exon 40) | TCAGTACAAGAGGCAGGCTG | NM_001012408 | 330 |
| DMD across SNP pig Rev (exon 42) | GGCATGTCTTCAGTCATCAC | ||
| DMD 3′ of SNP pig For (exon 41) | AATTTGCTCACTTTCGAAGA | NM_001012408 | 185 |
| DMD 3′ of SNP pig Rev (exon 42) | GAGGTCAGGAGCATTGAGAA | ||
| DMD 3′ pig For (exon 62/63) | CCACGAGACCCAAACAACTT | NM_001012408 | 153 |
| DMD 3′ pig Rev (exon 65) | AGGCTCAAGAGATCCAAGCA | ||
| TNF pig For | GCCCTTCCACCAACGTTTTC | NM_214022 | 158 |
| TNF pig Rev | TCCCAGGTAGATGGGTTCGT | ||
| IL1B pig For | AAGATAACACGCCCACCCTG | NM_214055 | 293 |
| IL1B pig Rev | TGTCAGCTTCGGGGTTCTTC | ||
| IL6 pig For | AGATGCCAAAGGTGATGCCA | NM_001252429 | 363 |
| IL6 pig Rev | CTCAGGGTCTGGATCAGTGC | ||
| MYOD1 pig For | CTACAGCGGTGACTCAGACG | NM_001002824 | 121 |
| MYOD1 pig Rev | GCTGTAATAGGTGCCGTCGT | ||
| DTNA pig For | ACTACCCACGGCAGTTTTTG | XM_003356399 | 110 |
| DTNA pig Rev | GCGTGTCCAAGAAACCATTT | ||
| SGCA pig For | AGGTCGAAAGGAAGGCGTAT | NM_001144122 | 131 |
| SGCA pig Rev | CATAGCAGGACAGCAGTGGA | ||
| NOS1 pig For | GGAAAACAGTCTCCCACCAA | XM_003132898 | 127 |
| NOS1 pig Rev | ATCCTGTTCCCAATGTGCTC | ||
| UTRN pig For | GAACGGATCATTGCTGACCT | XM_003121163 | 177 |
| UTRN pig Rev | CCTGAGGAGTTTGGCTTCTG | ||
| 18S RT primer | GAGCTGGAATTACCGCGGCT | ||
| 18S pig For | AAACGGCTACCACATCCAAG | NR_046261 | 141 |
| 18S pig Rev | TCGCGGAAGGATTTAAAGTG |
DTNA, α-dystrobrevin; For, forward; IL1B, interleukin 1β; IL6, interleukin 6; MYOD1, myogenic differentiation 1; NOS1, (neuronal) nitric oxide synthase 1; Rev, reverse; SGCA, α-sarcoglycan; TNF, tumor necrosis factor; UTRN, utrophin.
a
GeneBank dbSNP ss410758971.