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. 2014 Apr;28(4):1666–1681. doi: 10.1096/fj.13-240390

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Figure 1. — Generation of Sod1 neuron rescue Sod1^−/− mice (SynTgSod1^−/−). A) Schematic for generating Syn-hSOD1 transgenic mice and breeding to Sod1^−/− mice to generate SynTgSod1^−/− mice. B, C) Western blot analysis of SOD1 content of the AT (B) and GTN (C) muscles of Sod1^−/−, WT, and SynTgSod1^−/− mice. D, E) Western blot analysis of SOD1 (D) and SOD2 (E) content of sciatic nerve (SN) of Sod1^−/−, WT, and SynTgSod1^−/− mice and densitometric quantification of the blots. *P < 0.05 vs. WT. F) Western blot analysis of the SOD1 content of the spinal cord (SC) of WT, Sod1^−/−, and SynTgSod1^−/− mice and densitometric quantification of the blots. *P < 0.05 vs. WT. G) Representative Western blot of the SOD1 content of the liver of Sod1^−/−, WT, and SynTgSod1^−/− mice. H–J) Native gels stained for SOD1 and SOD2 enzyme activities in skeletal muscle (H), brain (I), and spinal cord (J) from WT, Sod1^−/−, and SynTgSod1^−/− mice. K) Transverse sections of lumbar spinal cord dorsal horn from WT, Sod1^−/−, and SynTgSod1^−/− mice labeled with human SOD1 (green) and GFAP (red), a marker of astrocytes. Nuclei (blue) were labeled with DAPI (×100).

Figure 1. — Generation of Sod1 neuron rescue Sod1^−/− mice (SynTgSod1^−/−). A) Schematic for generating Syn-hSOD1 transgenic mice and breeding to Sod1^−/− mice to generate SynTgSod1^−/− mice. B, C) Western blot analysis of SOD1 content of the AT (B) and GTN (C) muscles of Sod1^−/−, WT, and SynTgSod1^−/− mice. D, E) Western blot analysis of SOD1 (D) and SOD2 (E) content of sciatic nerve (SN) of Sod1^−/−, WT, and SynTgSod1^−/− mice and densitometric quantification of the blots. *P < 0.05 vs. WT. F) Western blot analysis of the SOD1 content of the spinal cord (SC) of WT, Sod1^−/−, and SynTgSod1^−/− mice and densitometric quantification of the blots. *P < 0.05 vs. WT. G) Representative Western blot of the SOD1 content of the liver of Sod1^−/−, WT, and SynTgSod1^−/− mice. H–J) Native gels stained for SOD1 and SOD2 enzyme activities in skeletal muscle (H), brain (I), and spinal cord (J) from WT, Sod1^−/−, and SynTgSod1^−/− mice. K) Transverse sections of lumbar spinal cord dorsal horn from WT, Sod1^−/−, and SynTgSod1^−/− mice labeled with human SOD1 (green) and GFAP (red), a marker of astrocytes. Nuclei (blue) were labeled with DAPI (×100).