Figure 5.

Depletion of satellite cells is associated with increased ECM deposition and fibroblast content 8 wk following SA surgery (SA-8). A) Plantaris muscle cross sections incubated with fluorescently labeled wheat germ agglutinin quantifies N-acetyl-d-glucosamine (red) a major component of the ECM. Scale bar = 100 μm. B) Quantification of area occupied by wheat germ agglutinin staining, presented as mean ± se area of wheat germ agglutinin per fiber; n = 4–6. C) Tcf4 immunohistochemistry (red) on plantaris muscle cross sections counterstained with DAPI (blue). Scale bar = 100 μm. D) Quantification of Tcf4⁺/DAPI⁺ fibroblasts, presented as mean ± se Tcf4⁺ fibroblasts per area of muscle (mm²); n = 6. *P < 0.05 for surgery between condition-matched groups; ^†P < 0.05 for tamoxifen between condition-matched groups.