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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: Oncogene. 2012 Jan 9;31(38):4245–4254. doi: 10.1038/onc.2011.586

Figure 6. Metnase phosphorylation regulates its biochemoical activities.

Figure 6

Metnase dimethylation of histone 3 lysine 35 (H3k36) and subsequent enhancement of DSB repair requires S495 phosphorylation. A. Western analysis of H3K36 dimethyltion (me2) by wt and S495A Metnase after DNA damage induced by hydroxyurea (HU). B. ChIP analysis (upper panel) over time after induction of a single DSB by ISCE-I for the adjacent presence of H3K36me2 with over-expression of wt or S495A Metnase (Fnu et al, 2011). The lower panel shows real time PCR assessment of the re-ligation of this induced single DSB over time. * P <0.05, ** p<0.01. C. Flap cleavage nuclease activities of pure isolated eukaryotic wt and S495A Metnase. The upper schematic diagrams the altered S495A flap nuclease activity. The size of the arrows correlates with the amount of endonucleolysis.