Fig. 1.

Binding of PKCδ to the C-terminus of Cx43. (A) Left, this model shows the structure of Cx43 with its four transmembrane domains, two extracellular loops, a cytoplasmic loop, and intracellular N-terminus and C-terminus. Right, the amino acid composition of the Cx43 CT (amino acids 227–382) is shown. Putative interaction domains for complex binding with PKCδ are indicated in bold. (B) Top, a schematic of the Cx43 CT GST fusion constructs used for the GST-pulldown assays is shown. The full length Cx43 is shown as a reference with amino acid number indicated above. The CX43 CT domain is indicated in black. The deleted domains are indicated by a white box containing an X. Bottom, GST-pulldown assay of the input and bead fractions of whole cell extracts from MC3T3 cells, following SDS-PAGE and western blotting with anti-PKCδ antibodies. “GST beads” refers to beads conjugated to GST alone, rather than a GST-coupled Cx43 CT construct.