Table 2. Effect of pre-IVM on nuclear maturation of IVG oocytes .
Pre-IVM | No. of oocytes (replicates) |
% of oocytes at each stage | ||||
GV | GVBD | M I | A I/T I | M II | ||
– | 85 (5) | 5.0 ± 7.2 | 2.1 ± 2.9 | 26.6 ± 9.4a | 15.8 ± 10.7a | 49.4 ± 10.4a |
+ | 99 (5) | 1.3 ± 2.8 | 0 | 15.9 ± 4.3b | 2.7 ± 6.1b | 80.1 ± 9.6b |
Control* | 120 (5) | 0 | 0 | 9.6 ± 2.9b | 1.7 ± 2.4b | 88.7 ± 1.9b |
a, b Values (mean ± SD) with different superscripts within columns are significantly different (P<0.05). * The oocytes collected from antral follicles (2 to 8 mm in diameter) served as in vivo-grown controls. GV, germinal vesicle; GVBD, germinal vesicle breakdown; M I, metaphase I; A I/T I, anaphase I/telophase I; M II, metaphase II.