Table 3. Effect of pre-IVM on fertilization of IVG oocytes.
Pre-IVM | No. of oocytes (replicates) |
% normal fertilization | % of polyspermy | % total fertilization | ||
% of ESH | % of 2PN | % of subtotal | ||||
– | 124 (5) | 8.7 ± 9.3 | 56.8 ± 12.4 | 65.6 ± 7.9a | 7.5 ± 5.1 | 73.1 ± 4.4a |
+ | 207 (7) | 14.0 ± 12.3 | 58.9 ± 16.3 | 73.0 ± 12.6a | 6.3 ± 3.0 | 79.7 ± 13.6ab |
Control* | 148 (6) | 20.6 ± 18.5 | 66.7 ± 17.3 | 87.3 ± 6.2b | 5.1 ± 4.6 | 93.1 ± 4.9b |
a, b Values (mean ± SD) with different superscripts within columns are significantly different (P<0.05). * The oocytes collected from antral follicles (2 to 8 mm in diameter) served as in vivo-grown controls. ESH, enlarged sperm head; 2PN, two pronuclei.