Figure 4.
A. 1H NMR of the imino regions of the human telomere sequence (AG3(T2AG3)3) and of AS1411 ((G2T)4TGT(G2T)3G2). The human telomere exhibits 23-24 GN1H signals, indicative of the presence of two quadruplex species. AS1411 yields an irresolvable mixture of GN1H resonances known to be the result of more than 8 quadruplex species. B. CD of the human telomere sequence and of AS1411. The CD data provides no indication of the polymorphism evident from the NMR data. Conventional wisdom would associate both sets of CD data with a single, parallel stranded species in solution.