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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: J Comp Neurol. 2012 Jul 1;520(10):2202–2217. doi: 10.1002/cne.23038

Figure 4.

Figure 4

Immunohistochemical labeling of synaptic endings using GlyT2 antibodies identifies inhibitory synaptic contacts (white asterisks, c) on a representative MSO cell body of a cat with normal hearing (a). The immunoprecipitate darkens the mitochondria and forms a thin, dark halo around SVs, clearly revealing unstained terminals (black asterisk, b). b: A typical GlyT2-immunonegative ending has round SVs and asymmetric postsynaptic densities (arrows) characteristic of excitatory synapses. c: A typical GlyT2-immunopositive terminal has pleomorphic SVs and symmetric postsynaptic densities (arrow), characteristic of inhibitory synapses. Scale bars = 500 nm.