Table II.
The proportion of senescent cells in normal and osteoarthritic (OA) chondrocyte cultures at 24 and 48 hours. Chondrocytes were incubated in 12-well plates with different concentrations of resveratrol (0, 1 µmol/l, 10 µmol/l, 25 µmol/l and 50 µmol/l) in four samples for each experiment
| Resveratrol concentration (µM) | ||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean (sd) proportion of senescent cells (%) | 0 | 1 | p-value* | 10 | p-value* | 25 | p-value* | 50 | p-value* | |||||||||
| 24 HOURS | ||||||||||||||||||
| Normal chondrocytes | 41.0 (5.8) | 33.0 (6.9) | 0.148 | 36.1 (1.9) | 0.509 | 56.4 (5.6) | 0.003 | 40.5 (6.1) | 1.00 | |||||||||
| OA chondrocytes | 48.8 (8.2) | 48.0 (4.9) | 0.999 | 51.2 (5.3) | 0.908 | 49.7 (3.0) | 0.996 | 42.5 (2.8) | 0.300 | |||||||||
| p-value | 0.171 | 0.012 | 0.002 | 0.048 | 0.565 | |||||||||||||
| 48 HOURS | ||||||||||||||||||
| Normal chondrocytes | 30.6 (14.0) | 34.9 (7.0) | 0.844 | 37.9 (4.5) | 0.503 | 44.4 (5.3) | 0.076 | 42.9 (3.2) | 0.123 | |||||||||
| OA chondrocytes | 31.5 (2.2) | 35.0 (4.3) | 0.533 | 42.3 (3.7) | 0.004 | 39.8 (3.6) | 0.028 | 44.2 (5.0) | 0.001 | |||||||||
| p-value | 0.898 | 0.987 | 0.179 | 0.197 | 0.691 | |||||||||||||
* compared with control cultures (0 µM concentration). Analysed using independent t-test