Table 1.
Liquid chromatography tandem mass spectrometry parameters for synthetic cannabinoids and metabolites in human urine.
| Analyte | Peak # | Q1 mass (amu) |
Q3 masses (amu) |
DP (V) |
EP (V) |
CE (V) |
CXP (V) |
RT (min) |
|---|---|---|---|---|---|---|---|---|
| A. Positive Mode Method | ||||||||
| JWH-018 | 42 | 342.1 | 155.2, 127.2 | 46 | 10 | 33, 53 | 12, 12 | 12.30 |
| JWH-018 5-hydroxyindole | 33 | 358.2 | 155.1, 127.2 | 91 | 10 | 33, 65 | 16, 10 | 10.70 |
| JWH-018 6-hydroxyindole | 29 | 358.1 | 155.1, 127.2 | 76 | 10 | 31, 69 | 16, 10 | 10.20 |
| JWH-018 N-5-hydroxypentyl | 16 | 358.2 | 155.1, 127.2 | 56 | 10 | 29, 65 | 12, 14 | 8.48 |
| JWH-018 N-pentanoic acid | 15 | 372.2 | 155.0, 126.9 | 106 | 10 | 31, 71 | 14, 14 | 8.47 |
| JWH-019 | 45 | 356.0 | 154.9, 127.0 | 26 | 10 | 33, 65 | 16, 14 | 12.90 |
| JWH-019 5-hydroxyindole | 36 | 372.0 | 155.0, 127.0 | 11 | 10 | 33, 71 | 12, 12 | 11.40 |
| JWH-019 N-6-hydroxyhexyl | 23 | 372.0 | 154.9, 127.0 | 86 | 10 | 29, 73 | 14, 12 | 9.34 |
| JWH-073 | 39 | 328.0 | 155.2, 127.1 | 51 | 10 | 31, 63 | 12, 14 | 11.70 |
| JWH-073 5-hydroxyindole | 27 | 344.2 | 155.0, 127.0 | 51 | 10 | 33, 65 | 12, 18 | 9.88 |
| JWH-073 6-hydroxyindole | 24 | 344.2 | 155.1, 127.0 | 46 | 10 | 31, 67 | 10, 14 | 9.37 |
| JWH-073 N-4-hydroxybutyl | 10 | 344.1 | 155.0, 127.0 | 36 | 10 | 29, 51 | 12, 18 | 7.68 |
| JWH-073 N-butanoic acid | 13 | 358.1 | 155.0, 127.0 | 61 | 10 | 31, 61 | 14, 12 | 7.80 |
| JWH-081 | 43 | 372.1 | 185.1, 157.2 | 181 | 10 | 33, 51 | 16, 10 | 12.70 |
| JWH-081 N-5-hydroxypentyl | 18 | 388.2 | 185.1, 113.9 | 56 | 10 | 29, 99 | 16, 14 | 9.09 |
| JWH-122 | 46 | 356.1 | 169.0, 115.0 | 51 | 10 | 33, 91 | 16, 12 | 12.90 |
| JWH-122 N-5-hydroxypentyl | 22 | 372.1 | 169.0, 115.0 | 51 | 10 | 29, 85 | 12, 14 | 9.29 |
| JWH-200 | 5 | 385.0 | 155.1, 126.8 | 126 | 10 | 29, 69 | 10, 14 | 5.41 |
| JWH-200 5-hydroxyindole | 1 | 401.1 | 155.0, 76.9 | 31 | 10 | 29, 125 | 14, 8 | 2.99 |
| JWH-200 6-hydroxyindole | 2 | 401.1 | 155.0, 127.0 | 120 | 10 | 29, 71 | 12, 16 | 3.48 |
| JWH-203 | 38 | 340.9 | 124.9, 89.1 | 6 | 10 | 35, 103 | 14, 8 | 11.50 |
| JWH-210 | 48 | 370.1 | 183.1, 214.1 | 51 | 10 | 33, 33 | 18, 18 | 13.50 |
| JWH-210 5-hydroxyindole | 41 | 386.2 | 183.0, 155.1 | 51 | 10 | 35, 49 | 14, 14 | 12.20 |
| JWH-210 N-5-hydroxypentyl | 30 | 386.2 | 183.1, 155.1 | 30 | 10 | 31, 47 | 16, 12 | 10.20 |
| JWH-210 N-5-carboxypentyl | 28 | 400.1 | 183.0, 155.0 | 40 | 10 | 33, 49 | 14, 18 | 10.10 |
| JWH-250 | 35 | 336.1 | 121.2, 91.1 | 50 | 10 | 27, 61 | 12, 14 | 11.10 |
| JWH-250 5-hydroxyindole | 20 | 352.1 | 121.0, 91.0 | 66 | 10 | 27, 65 | 14, 12 | 9.19 |
| JWH-250 N-5-hydroxypentyl | 8 | 352.2 | 121.0, 186.2 | 50 | 10 | 27, 21 | 12, 14 | 7.02 |
| JWH-250 N-5-carboxypentyl | 9 | 366.1 | 121.1, 200.1 | 56 | 10 | 27, 23 | 14, 16 | 7.04 |
| JWH-398 | 47 | 377.0 | 188.8, 126.0 | 81 | 10 | 33, 97 | 22, 16 | 13.20 |
| JWH-398 N-5-hydroxypentyl | 26 | 393.1 | 188.8, 160.9 | 51 | 10 | 29, 59 | 18, 14 | 9.85 |
| JWH-398 N-pentanoic acid | 25 | 406.9 | 189.1, 161.1 | 31 | 10 | 31, 65 | 18, 14 | 9.82 |
| AM2201 | 37 | 360.1 | 155.1, 127.2 | 106 | 10 | 33, 57 | 10, 10 | 11.40 |
| AM2201 6-hydroxyindole | 19 | 376.2 | 127.1, 77.0 | 66 | 10 | 67, 111 | 10, 12 | 9.09 |
| AM2201 N-4-hydroxypentyl | 14 | 376.1 | 155.0, 126.9 | 46 | 10 | 33, 69 | 14, 18 | 8.22 |
| MAM2201 | 40 | 374.1 | 169.0, 115.0 | 30 | 10 | 35, 91 | 14, 14 | 12.00 |
| MAM2201 N-4-hydroxypentyl | 17 | 390.1 | 169.0, 141.1 | 166 | 10 | 35, 59 | 18, 12 | 9.02 |
| MAM2201 N-pentanoic acid | 21 | 386.1 | 169.1, 141.1 | 61 | 10 | 33, 49 | 20, 16 | 9.26 |
| AM694 | 31 | 435.9 | 230.8, 202.9 | 196 | 10 | 35, 61 | 18, 20 | 10.40 |
| RCS-4 | 34 | 322.1 | 135.1, 77.2 | 56 | 10 | 31, 73 | 12, 10 | 10.70 |
| RCS-4 N-5-hydroxypentyl | 6 | 338.2 | 135.0, 77.0 | 141 | 10 | 27, 73 | 12, 12 | 6.47 |
| RCS-4 N-5-carboxypentyl | 7 | 352.1 | 135.0, 107.0 | 51 | 10 | 31, 59 | 14, 12 | 6.49 |
| RCS-4 M9 metabolite | 3 | 324.1 | 120.9, 92.9 | 21 | 10 | 27, 63 | 14, 14 | 4.09 |
| RCS-4 M10 metabolite | 4 | 324.1 | 120.9, 93.0 | 16 | 10 | 31, 63 | 14, 14 | 4.31 |
| RCS8 | 44 | 376.0 | 120.9, 90.9 | 146 | 10 | 31, 65 | 10, 12 | 12.80 |
| UR-144 N-5-hydroxypentyl | 11 | 328.0 | 124.9, 97.0 | 141 | 10 | 25, 37 | 18, 14 | 7.78 |
| UR-144 N-pentanoic acid | 12 | 342.0 | 125.0, 244.1 | 61 | 10 | 27, 31 | 10, 18 | 7.78 |
| XLR11 | 32 | 330.1 | 125.1, 232.0 | 156 | 10 | 31, 33 | 6, 24 | 10.50 |
| JWH-018-d9 | 351.1 | 155.0, 127.0 | 36 | 10 | 33, 53 | 14, 16 | 12.30 | |
| JWH-018 5-hydroxyindole-d9 | 367.1 | 155.0, 127.0 | 56 | 10 | 35, 73 | 18, 12 | 10.60 | |
| JWH-018 6-hydroxyindole-d9 | 367.1 | 155.0, 127.0 | 46 | 10 | 33, 73 | 14, 14 | 10.20 | |
| JWH-018 N-5-hydroxypentyl-d5 | 363.1 | 155.0, 127.0 | 56 | 10 | 29, 65 | 14, 14 | 8.43 | |
| JWH-073-d7 | 335.1 | 155.0, 127.0 | 76 | 10 | 33, 65 | 14, 16 | 11.60 | |
| JWH-073 5-hydroxyindole-d7 | 351.1 | 155.0, 127.0 | 51 | 10 | 33, 51 | 12, 16 | 9.81 | |
| JWH-073 6-hydroxyindole-d7 | 351.1 | 155.0, 127.0 | 36 | 10 | 33, 61 | 12, 10 | 9.31 | |
| JWH-073 N-4-hydroxybutyl-d5 | 349.1 | 155.0, 127.0 | 46 | 10 | 29, 57 | 14, 14 | 7.62 | |
| JWH-073 N-butanoic acid-d5 | 363.1 | 155.0, 127.0 | 61 | 10 | 31, 61 | 12, 16 | 7.74 | |
| JWH-081-d9 | 381.2 | 185.0, 157.0 | 51 | 10 | 35, 53 | 18, 12 | 12.70 | |
| JWH-122-d9 | 365.2 | 169.0, 114.9 | 86 | 10 | 35, 95 | 16, 14 | 12.90 | |
| JWH-122 N-5-hydroxypentyl-d5 | 377.1 | 169.0, 114.9 | 56 | 10 | 29, 97 | 16, 14 | 9.23 | |
| JWH-200-d5 | 390.1 | 155.1, 127.0 | 41 | 10 | 29, 65 | 14, 18 | 5.35 | |
| JWH-210-d9 | 379.2 | 183.0, 223.1 | 36 | 10 | 33, 33 | 18, 18 | 13.40 | |
| JWH-250-d5 | 341.1 | 121.0, 91.0 | 46 | 10 | 27, 63 | 14, 12 | 11.00 | |
| JWH-398-d9 | 385.9 | 189.0, 125.9 | 36 | 10 | 37, 93 | 20, 18 | 13.10 | |
| AM2201-d5 | 365.1 | 155.0, 127.0 | 31 | 10 | 35, 73 | 14, 12 | 11.30 | |
| AM2201 N-4-hydroxypentyl-d5 | 381.1 | 155.1, 127.1 | 50 | 10 | 29, 77 | 16, 16 | 8.15 | |
| RCS-4-d9 | 331.1 | 135.0, 77.0 | 61 | 10 | 33, 75 | 12, 10 | 10.60 | |
| UR-144-d5 | 317.0 | 125.0, 218.6 | 61 | 10 | 31, 33 | 12, 18 | 11.40 | |
| XLR11-d5 | 335.1 | 125.1, 237.1 | 161 | 10 | 31, 33 | 12, 20 | 10.40 | |
| B. Negative Mode Method | ||||||||
| CP 47,497-C7 | 51 | 317.1 | 245.1, 159.1 | −90 | −10 | −42, −64 | −29, −15 | 6.26 |
| CP 47,497-C7-hydroxy dimethylheptyl metabolite | 49 | 333.2 | 261.2, 158.9 | −15 | −10 | −50, −76 | −15, −15 | 4.49 |
| CP 47,497-C8 | 52 | 331.1 | 259.1, 159.0 | −30 | −10 | −46, −68 | −23, −15 | 6.54 |
| CP 47,497-C8-hydroxy dimethyloctyl metabolite | 50 | 347.1 | 159.1, 185.0 | −180 | −10 | −72, −66 | −17, −19 | 4.91 |
| HU210 | 53 | 385.6 | 301.3, 281.1 | −36 | −12 | −48, −58 | −27, −26 | 6.93 |
| CP 47,497-C7-d11 | 328.2 | 256.2, 159.0 | −160 | −10 | −44, −68 | −23, −15 | 6.22 | |
| CP 47,497-C8-d7 | 338.2 | 266.1, 159.0 | −150 | −10 | −46, −64 | −33, −17 | 6.52 | |
| 11-nor-9-carboxy-tetrahydrocannabinol-d9 | 352.1 | 254.2, 194.1 | −50 | −10 | −40, −44 | −21, −17 | 6.14 |
Q1= quadrupole 1, Q3= quadrupole 3, DP= declustering potential, EP= entrance potential, CE= collision energy, CXP= collision cell exit potential, RT= retention time.
Bold masses depict quantification transitions.