Table 2.
Analytical recovery and imprecision data for synthetic cannabinoids and metabolites in human urine by liquid chromatography tandem mass spectrometry.
| Analyte | Target Low µg/l |
Intra-day, N=4 |
Inter-day, N=20 |
||||
|---|---|---|---|---|---|---|---|
| Accuracy (%CV) | Accuracy (%CV) | ||||||
| Low | Mid | High | Low | Mid | High | ||
| JWH-018 | 0.6 | 109.2 (8.1) | 102.6 (7.9) | 101.6 (9.8) | 102.3 (7.6) | 103.4 (7.6) | 101.2 (8.1) |
| JWH-018 5-hydroxyindole | 0.3 | 109.2 (6.3) | 108.1 (8.7) | 106.6 (5.0) | 107.2 (7.9) | 108.1 (6.9) | 103.8 (7.5) |
| JWH-018 6-hydroxyindole | 0.3 | 104.2 (4.8) | 107.5 (6.8) | 106.0 (7.4) | 106.2 (7.3) | 110.4 (6.5) | 102.0 (10.0) |
| JWH-018 N-hydroxypentyl | 0.3 | 97.5 (12.9) | 98.9 (7.7) | 96.1 (7.0) | 96.0 (9.4) | 102.4 (7.2) | 92.8 (7.6) |
| JWH-018 N-pentanoic acid | 0.3 | 110.8 (9.3) | 112.4 (3.1) | 109.8 (4.7) | 111.0 (6.4) | 112.0 (4.3) | 109.6 (5.2) |
| JWH-019 | 0.3 | 104.2 (11.5) | 100.2 (7.8) | 100.2 (10.7) | 101.3 (10.8) | 103.3 (7.9) | 99.6 (9.2) |
| JWH-019 5-hydroxyindole | 0.3 | 100.8 (11.2) | 99.8 (5.9) | 96.7 (8.6) | 100.0 (11.7) | 103.6 (10.0) | 96.3 (7.2) |
| JWH-019 N-hydroxyhexyl | 0.3 | 111.7 (5.2) | 111.8 (3.2) | 107.3 (5.2) | 107.5 (7.9) | 112.0 (4.4) | 105.6 (7.4) |
| JWH-073 | 0.3 | 108.3 (8.1) | 100.7 (8.5) | 100.2 (6.1) | 98.2 (10.1) | 101.4 (8.6) | 97.3 (7.1) |
| JWH-073 5-hydroxyindole | 0.3 | 105.0 (9.9) | 106.0 (8.8) | 105.3 (5.3) | 103.5 (8.1) | 107.6 (7.4) | 100.7 (7.7) |
| JWH-073 6-hydroxyindole | 0.3 | 102.5 (9.3) | 99.3 (9.8) | 99.7 (7.1) | 100.7 (8.8) | 103.0 (7.6) | 96.0 (8.2) |
| JWH-073 N-hydroxybutyl | 0.3 | 105.8 (4.0) | 105.4 (6.7) | 101.6 (5.7) | 102.7 (8.6) | 106.5 (7.8) | 97.6 (5.9) |
| JWH-073 N-butanoic acid | 0.3 | 102.5 (9.7) | 103.8 (8.7) | 101.4 (7.0) | 105.5 (7.8) | 105.4 (6.7) | 99.0 (5.3) |
| JWH-081 | 0.3 | 105.8 (6.5) | 102.0 (7.8) | 99.8 (9.4) | 98.8 (9.4) | 104.1 (7.2) | 96.4 (7.5) |
| JWH-081 N-hydroxypentyl | 0.3 | 112.5 (7.8) | 107.8 (6.4) | 104.7 (2.2) | 108.2 (8.1) | 111.1 (5.6) | 104.6 (7.0) |
| JWH-122 | 0.3 | 102.5 (6.7) | 95.5 (7.2) | 90.0 (8.1) | 101.3 (9.8) | 105.4 (8.7) | 98.5 (8.7) |
| JWH-122 N-hydroxypentyl | 0.3 | 101.7 (11.2) | 100.9 (9.2) | 99.3 (4.0) | 99.7 (8.3) | 103.7 (7.7) | 97.4 (7.3) |
| JWH-200 | 0.3 | 103.3 (9.5) | 102.0 (9.4) | 98.4 (6.6) | 99.3 (9.1) | 103.1 (6.8) | 97.5 (6.7) |
| JWH-200 5-hydroxyindole | 1.5 | 109.2 (6.6) | 107.2 (7.7) | 108.7 (5.0) | 104.5 (6.4) | 104.1 (7.5) | 103.2 (7.6) |
| JWH-200 6-hydroxyindole | 0.3 | 110.0 (6.5) | 107.3 (9.3) | 108.7 (3.4) | 104.3 (8.5) | 106.0 (7.9) | 104.8 (6.1) |
| JWH-203 | 1.5 | 94.0 (7.7) | 87.9 (3.8) | 88.8 (5.4) | 98.0 (9.9) | 96.8 (10.7) | 96.2 (10.0) |
| JWH-210 | 0.3 | 110.0 (2.5) | 102.9 (10.0) | 101.2 (7.5) | 105.0 (8.0) | 106.3 (8.6) | 101.5 (7.5) |
| JWH-210 5-hydroxyindole | 0.6 | 100.0 (10.4) | 94.3 (6.9) | 94.5 (9.2) | 98.7 (10.7) | 106.8 (7.8) | 104.6 (8.4) |
| JWH-210 N-hydroxypentyl | 0.3 | 96.7 (13.5) | 95.5 (7.1) | 93.9 (10.0) | 97.8 (9.3) | 101.8 (9.2) | 91.3 (7.6) |
| JWH-210 N-5-carboxypentyl | 0.3 | 104.2 (13.7) | 103.2 (3.3) | 96.2 (8.0) | 99.7 (10.3) | 103.8 (6.8) | 97.6 (7.2) |
| JWH-250 | 0.6 | 107.5 (5.1) | 101.6 (7.9) | 101.3 (5.6) | 104.9 (7.3) | 103.9 (8.3) | 98.7 (6.9) |
| JWH-250 5-hydroxyindole | 0.3 | 110.0 (6.5) | 103.5 (7.1) | 104.7 (8.0) | 105.7 (7.3) | 107.3 (7.0) | 103.6 (8.3) |
| JWH-250 N-hydroxypentyl | 0.6 | 108.8 (5.8) | 109.3 (7.6) | 104.5 (9.5) | 106.6 (6.1) | 107.9 (6.6) | 99.1 (9.4) |
| JWH-250 N-5-carboxypentyl | 0.6 | 115.4 (1.4) | 111.5 (8.7) | 111.8 (10.9) | 111.3 (5.4) | 111.4 (6.7) | 105.2 (9.8) |
| JWH-398 | 1.5 | 98.3 (10.9) | 102.6 (7.9) | 101.4 (2.4) | 100.1 (10.0) | 105.9 (8.2) | 101.7 (11.2) |
| JWH-398 N-hydroxypentyl | 1.5 | 112.5 (4.5) | 105.3 (6.2) | 101.1 (5.1) | 105.2 (8.5) | 106.1 (5.4) | 102.0 (6.1) |
| JWH-398 N-pentanoic acid | 1.5 | 99.7 (9.1) | 101.0 (9.7) | 99.4 (5.9) | 95.2 (10.2) | 93.6 (7.6) | 92.5 (7.2) |
| AM2201 | 0.3 | 105.0 (8.4) | 105.0 (9.6) | 104.1 (5.6) | 105.3 (7.1) | 107.9 (6.0) | 102.6 (7.3) |
| AM2201 6-hydroxyindole | 1.5 | 109.2 (7.8) | 107.1 (8.4) | 106.8 (6.6) | 109.1 (6.7) | 107.2 (6.9) | 104.4 (8.2) |
| AM2201 N-hydroxypentyl | 0.3 | 96.7 (12.95) | 98.1 (10.0) | 96.6 (6.5) | 94.2 (10.0) | 99.7 (9.2) | 93.3 (6.3) |
| MAM2201 | 0.3 | 96.7 (9.8) | 97.7 (5.1) | 88.9 (3.3) | 92.0 (7.7) | 94.0 (6.3) | 88.3 (5.3) |
| MAM2201 N-hydroxypentyl | 0.3 | 110.0 (10.5) | 110.0 (5.6) | 107.3 (3.4) | 104.7 (9.4) | 110.4 (5.5) | 105.3 (5.6) |
| MAM2201 N-pentanoic acid | 0.6 | 104.6 (9.4) | 105.9 (6.5) | 101.9 (5.6) | 103.1 (8.1) | 105.6 (6.6) | 101.2 (6.7) |
| AM694 | 0.3 | 97.5 (12.3) | 103.5 (7.0) | 102.2 (8.0) | 100.8 (11.2) | 107.3 (6.4) | 103.9 (7.5) |
| RCS-4 | 0.3 | 106.7 (8.5) | 105.1 (11.8) | 101.2 (6.2) | 104.7 (8.5) | 108.4 (7.6) | 101.6 (8.4) |
| RCS-4 N-hydroxypentyl | 0.6 | 105.8 (6.9) | 102.8 (7.7) | 99.8 (8.7) | 102.2 (8.3) | 105.6 (8.1) | 96.7 (7.4) |
| RCS-4 N-5-carboxypentyl | 0.6 | 118.3 (1.1) | 111.8 (6.8) | 110.9 (10.2) | 112.2 (5.7) | 111.7 (6.1) | 106.7 (8.2) |
| RCS-4 M9 metabolite | 0.6 | 112.5 (4.4) | 110.0 (8.4) | 107.8 (7.6) | 105.9 (7.5) | 108.9 (6.6) | 104.1 (6.1) |
| RCS-4 M10 metabolite | 0.6 | 112.1 (6.5) | 109.7 (8.7) | 107.3 (7.2) | 105.9 (7.9) | 109.4 (7.0) | 103.3 (6.6) |
| RCS8 | 0.3 | 110.0 (10.2) | 103.6 (11.7) | 98.9 (7.3) | 97.5 (12.1) | 99.0 (10.7) | 92.7 (8.6) |
| UR-144 N-hydroxypentyl | 0.3 | 95.0 (14.2) | 91.6 (8.5) | 88.0 (6.9) | 90.0 (9.3) | 97.7 (10.3) | 88.5 (5.5) |
| UR-144 N-pentanoic acid | 0.6 | 100.8 (10.3) | 96.7 (8.6) | 94.2 (4.2) | 103.6 (8.0) | 102.9 (8.4) | 97.0 (5.2) |
| XLR11 | 0.6 | 101.7 (5.8) | 100.6 (6.0) | 100.6 (6.5) | 101.7 (7.1) | 101.7 (9.2) | 97.2 (9.7) |
| CP 47,497-C7 | 1.5 | 113.7 (4.2) | 103.0 (6.4) | 95.6 (5.9) | 106.2 (9.4) | 106.4 (7.1) | 97.4 (7.3) |
| CP 47,497-C7-hydroxy metabolite | 1.5 | 105.8 (12.1) | 112.6 (7.0) | 111.0 (7.4) | 103.7 (10.2) | 108.8 (9.4) | 105.2 (10.4) |
| CP 47,497-C8 | 1.5 | 112.7 (5.6) | 105.3 (8.0) | 105.1 (7.8) | 106.2 (9.1) | 105.4 (7.1) | 103.1 (6.8) |
| CP 47,497-C8-hydroxy metabolite | 1.5 | 101.0 (16.8) | 93.6 (9.1) | 95.7 (5.2) | 99.0 (13.5) | 99.9 (12.2) | 98.1 (11.0) |
| HU210 | 1.5 | 95.5 (9.7) | 95.9 (12.7) | 86.5 (6.0) | 97.3 (10.8) | 101.0 (11.7) | 99.2 (11.8) |
| % target (range) | 94.0 – 118.3 | 87.9 – 112.6 | 86.5 – 111.8 | 90.0 – 112.2 | 93.6 – 112.1 | 88.3 – 109.6 | |
| %CV (range) | 1.1 – 16.8 | 3.1 – 12.7 | 2.2 – 10.9 | 5.4 – 13.5 | 4.3 – 12.2 | 5.2 – 11.8 | |
Mid and high quality control concentrations were 7.5 and 30 µg/l, respectively.