Table 3.
Synthetic cannabinoids and metabolites in human urine by liquid chromatography tandem mass spectrometry: limits of detection (LOD), linear ranges and calibration results (N=6).
| Analyte | Internal Standard |
LOD (µg/l) |
Linear range (µg/l) |
Y-intercept Mean (SD) |
Slope Mean (SD) |
R2 (range) |
|---|---|---|---|---|---|---|
| JWH-018 | JWH-018-d9 | 0.2 | 0.2–50 | 0.78 (0.03) | 0.02 (0.01) | 0.995–0.999 |
| JWH-018 5-hydroxyindole | JWH-018 5-hydroxyindole-d9 | 0.1 | 0.1–50 | 1.08 (0.06) | 0.02 (0.01) | 0.994–0.998 |
| JWH-018 6-hydroxyindole | JWH-018 6-hydroxyindole-d9 | 0.1 | 0.1–50 | 0.96 (0.06) | 0.02 (0.01) | 0.994–0.998 |
| JWH-018 N-hydroxypentyl | JWH-018 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 1.19 (0.04) | 0.04 (0.01) | 0.996–0.997 |
| JWH-018 N-pentanoic acid | JWH-018 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 0.71 (0.04) | 0.01 (0.01) | 0.996–0.999 |
| JWH-019 | JWH-018-d9 | 0.1 | 0.1–50 | 0.63 (0.06) | 0.01 (0.01) | 0.995–0.999 |
| JWH-019 5-hydroxyindole | JWH-018 5-hydroxyindole-d9 | 0.1 | 0.1–50 | 0.95 (0.11) | 0.02 (0.01) | 0.995–0.997 |
| JWH-019 N-hydroxyhexyl | JWH-122 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 1.04 (0.05) | 0.03 (0.01) | 0.995–0.998 |
| JWH-073 | JWH-073-d7 | 0.1 | 0.1–50 | 0.48 (0.02) | 0.01 (0.01) | 0.995–0.998 |
| JWH-073 5-hydroxyindole | JWH-073 5-hydroxyindole-d7 | 0.1 | 0.1–50 | 0.94 (0.06) | 0.02 (0.01) | 0.995–0.998 |
| JWH-073 6-hydroxyindole | JWH-073 6-hydroxyindole-d7 | 0.1 | 0.1–50 | 0.94 (0.06) | 0.02 (0.01) | 0.995–0.998 |
| JWH-073 N-hydroxybutyl | JWH-073 N-hydroxybutyl-d5 | 0.1 | 0.1–50 | 1.34 (0.11) | 0.02 (0.01) | 0.995–0.998 |
| JWH-073 N-butanoic acid | JWH-073 N-butanoic acid-d5 | 0.1 | 0.1–50 | 1.43 (0.08) | 0.02 (0.02) | 0.996–0.998 |
| JWH-081 | JWH-081-d9 | 0.1 | 0.1–50 | 0.78 (0.04) | 0.05 (0.01) | 0.994–0.998 |
| JWH-081 N-hydroxypentyl | JWH-122 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 1.06 (0.07) | 0.02 (0.01) | 0.995–0.997 |
| JWH-122 | JWH-122-d9 | 0.1 | 0.1–50 | 1.05 (0.08) | 0.02 (0.01) | 0.995–0.997 |
| JWH-122 N-hydroxypentyl | JWH-122 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 0.99 (0.05) | 0.02 (0.01) | 0.995–0.997 |
| JWH-200 | JWH-200-d5 | 0.1 | 0.1–50 | 8.01 (0.38) | 0.12 (0.05) | 0.996–0.997 |
| JWH-200 5-hydroxyindole | JWH-073 6-hydroxyindole-d7 | 1.0 | 1–100 | 0.77 (0.30) | −0.01 (0.03) | 0.994–0.999 |
| JWH-200 6-hydroxyindole | JWH-073 6-hydroxyindole-d7 | 0.1 | 0.1–50 | 0.81 (0.30) | 0.01 (0.01) | 0.996–0.998 |
| JWH-203 | UR-144-d5 | 0.5 | 0.5–50 | 0.37 (0.04) | 0.08 (0.05) | 0.994–0.998 |
| JWH-210 | JWH-210-d9 | 0.1 | 0.1–50 | 1.30 (0.05) | 0.02 (0.02) | 0.995–0.999 |
| JWH-210 5-hydroxyindole | JWH-018-d9 | 0.2 | 0.2–50 | 1.24 (0.45) | 0.09 (0.06) | 0.995–0.999 |
| JWH-210 N-hydroxypentyl | JWH-018 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 1.38 (0.06) | 0.03 (0.01) | 0.994–0.997 |
| JWH-210 N-5-carboxypentyl | JWH-122 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 0.91 (0.09) | 0.01 (0.01) | 0.996–0.998 |
| JWH-250 | JWH-250-d5 | 0.2 | 0.2–50 | 0.61 (0.04) | 0.02 (0.02) | 0.995–0.998 |
| JWH-250 5-hydroxyindole | JWH-073 6-hydroxyindole-d7 | 0.1 | 0.1–50 | 0.77 (0.02) | 0.01 (0.004) | 0.995–0.998 |
| JWH-250 N-hydroxypentyl | JWH-073 N-hydroxybutyl-d5 | 0.2 | 0.2–50 | 1.41 (0.09) | 0.08 (0.02) | 0.995–0.997 |
| JWH-250 N-5-carboxypentyl | JWH-073 N-butanoic acid-d5 | 0.2 | 0.2–50 | 1.34 (0.09) | 0.03 (0.03) | 0.995–0.999 |
| JWH-398 | JWH-398-d9 | 0.5 | 0.5–50 | 0.93 (0.07) | 0.06 (0.07) | 0.996–0.999 |
| JWH-398 N-hydroxypentyl | JWH-122 N-hydroxypentyl-d5 | 0.5 | 0.5–50 | 0.08 (0.005) | 0.01 (0.004) | 0.995–0.999 |
| JWH-398 N-pentanoic acid | JWH-122 N-hydroxypentyl-d5 | 1.0 | 1–100 | 0.05 (0.006) | 0.01 (0.007) | 0.996–0.999 |
| AM2201 | AM2201-d5 | 0.1 | 0.1–50 | 7.60 (0.26) | 0.10 (0.03) | 0.994–0.999 |
| AM2201 6-hydroxyindole | JWH-073 6-hydroxyindole-d7 | 1.0 | 1–100 | 0.56 (0.04) | −0.02 (0.04) | 0.996–0.999 |
| AM2201 N-hydroxypentyl | AM2201 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 1.33 (0.08) | 0.03 (0.01) | 0.995–0.998 |
| MAM2201 | AM2201-d5 | 0.1 | 0.1–50 | 10.14 (2.22) | 0.22 (0.11) | 0.995–0.996 |
| MAM2201 N-hydroxypentyl | JWH-122 N-hydroxypentyl-d5 | 0.1 | 0.1–50 | 0.68 (0.03) | 0.01 (0.004) | 0.995–0.999 |
| MAM2201 N-pentanoic acid | JWH-122 N-hydroxypentyl-d5 | 0.2 | 0.2–50 | 0.82 (0.07) | 0.02 (0.01) | 0.995–0.999 |
| AM694 | XLR11-d5 | 0.1 | 0.1–50 | 1.99 (0.52) | 0.05 (0.03) | 0.996–0.998 |
| RCS-4 | RCS-4-d9 | 0.1 | 0.1–50 | 0.73 (0.04) | 0.01 (0.004) | 0.995–0.997 |
| RCS-4 N-hydroxypentyl | JWH-073 N-hydroxybutyl-d5 | 0.2 | 0.2–50 | 1.27 (0.09) | 0.04 (0.01) | 0.996–0.998 |
| RCS-4 N-5-carboxypentyl | JWH-073 N-butanoic acid-d5 | 0.2 | 0.2–50 | 1.39 (0.06) | 0.03 (0.02) | 0.995–0.997 |
| RCS-4 M9 metabolite | JWH-073 N-hydroxybutyl-d5 | 0.2 | 0.2–50 | 0.77 (0.13) | 0.03 (0.02) | 0.995–0.998 |
| RCS-4 M10 metabolite | JWH-073 N-hydroxybutyl-d5 | 0.2 | 0.2–50 | 0.77 (0.13) | 0.03 (0.02) | 0.995–0.998 |
| RCS8 | RCS-4-d9 | 0.1 | 0.1–50 | 0.46 (0.05) | 0.01 (0.01) | 0.995–0.998 |
| UR-144 N-hydroxypentyl | JWH-073 N-butanoic acid-d5 | 0.2 | 0.2–50 | 1.91 (0.17) | 0.06 (0.03) | 0.994–0.997 |
| UR-144 N-pentanoic acid | JWH-073 N-butanoic acid-d5 | 0.2 | 0.2–50 | 1.30 (0.07) | 0.05 (0.02) | 0.995–0.998 |
| XLR11 | XLR11-d5 | 0.2 | 0.2–50 | 1.32 (0.03) | 0.03 (0.01) | 0.995–0.999 |
| CP 47,497-C7 | CP 47,497-C7-d11 | 0.5 | 0.5–50 | 1.10 (0.06) | 0.11 (0.07) | 0.995–0.998 |
| CP 47,497-C7-hydroxy metabolite | 11-nor-9-tetrahydrocannabinol-d9 | 0.5 | 0.5–50 | 1.50 (0.46) | 0.21 (0.16) | 0.995–0.999 |
| CP 47,497-C8 | CP 47,497-C8-d7 | 0.5 | 0.5–50 | 0.75 (0.07) | 0.02 (0.02) | 0.995–0.999 |
| CP 47,497-C8-hydroxy metabolite | 11-nor-9-tetrahydrocannabinol-d9 | 0.5 | 0.5–50 | 2.21 (0.61) | 0.10 (0.16) | 0.994–0.999 |
| HU210 | CP 47,497-C8-d7 | 0.5 | 0.5–50 | 0.09 (0.02) | 0.03 (0.01) | 0.996–0.998 |
Limit of quantification was the lower limit of linearity.