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. Author manuscript; available in PMC: 2014 Mar 24.
Published in final edited form as: Immunol Rev. 2012 Nov;250(1):49–60. doi: 10.1111/imr.12004

Fig. 5. Replacement of TCRβ Y48 with an alanine in mice expressing a Vβ8-containing TCRβ as their only TCRβ chain severely reduces positive selection of T cells.

Fig. 5

A TCRβ chain containing the WT DO11.10 TCRβ chain or the same chain with Y46 or Y48 replaced by alanines (Y46A or Y48A) was introduced into C57BL/6 embryos. The mice so generated were crossed to mice deficient in TCRβ (108) and intercrossed to produce mice that could make only the transgenic TCRβ and any TCRα. Thymi in these mice were analyzed for their content of mature CD4+ and CD8+ cells. Shown are the means and SEMs of at least three mice of each type.