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. 2013 Sep 12;14(11):1166–1181. doi: 10.1111/tra.12102

Figure 2.

Figure 2

TgStx6 compartment replicates with the Golgi and ELC prior to nuclear division. A) ddStx6 parasites transiently transfected with GRASP-RFP to visualize the Golgi and stained with antibodies against the IMC (blue). (i and iv) Prior to replication, parasites have one Golgi and one TgStx6 compartment. (ii and v) The TgStx6 compartment replicates with the Golgi. White arrow indicates a parasite with two Golgi and two TgStx6 compartments. (iii) Newly forming daughter parasites contain two Golgi and two TgStx6 compartments. B) ddStx6 parasites stably transfected with IMC-tomato. Division of the TgStx6 compartment occurs prior to nuclear division. (i) Parasites with one nucleus and one TgStx6 compartment. (ii) TgStx6 compartment division. White arrow points to cell with one nucleus and two TgStx6 compartments. (iii) Nuclear division with two TgStx6 compartments. Daughter parasite IMC is clearly visible within the mother. (iv) After one division there are two daughter cells with two TgStx6 compartments and two nuclei. C) ddStx6 parasites stained with DAPI and antibodies against VP1, which indicate the ELC. (i) The TgStx6 compartment also replicates with the ELC. Replication is not synchronous across parasites within one PV. White arrow indicates a parasite with two ELC and two TgStx6 compartments. (ii) Prior to nuclear division, each parasite has two TGN and two ELC. (iii) After endodyogeny, each parasite contains one TgStx6 compartment and one ELC. Scale bars indicate 5 μM. D) Schematic of Golgi (magenta), TgStx6 compartment (green), IMC (purple), ELC (yellow) and nuclear (blue) replication.