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. 2013 Aug 14;90(1):6–21. doi: 10.1111/mmi.12342

Figure 3.

Figure 3

Transcription of degU and aprE is increased alongside total protease activity in ΔmotB.A. β-Galactosidase assays of strains carrying the PdegU–lacZ transcriptional reporter fusion. Strains shown are WT (wild-type NRS4351), ΔmotB (NRS4345), motB + amyE::PIPTG-motB-lacI (NRS4396) grown in the absence or presence of 50 μM IPTG, ΔmotAB (NRS4354) and ΔdegU (NRS4373). All cells were collected at the onset of stationary phase.B. β-Galactosidase assays of strains carrying the PaprE–lacZ transcriptional reporter fusion. Strains shown are WT (wild-type NRS1561), ΔmotB (NRS3440), ΔmotB + amyE::PIPTG-motB-lacI (NRS3858) grown in the absence or presence of 50 μM IPTG and ΔmotAB (NRS4093). All cells were collected at the onset of stationary phase.C. Total protease activity assays performed with the supernatant collected from cells grown in (B).Data in parts (A), (B) and (C) are plotted as the average of at least three independent replicates; in (C) data are represented as a fold change relative to the wild-type strain which was assigned value of 1. Error bars represent standard error of the mean. An asterisk denotes significance as calculated by the Student's t-test where * represents P < 0.05, ** P < 0.01 and *** P < 0.001.