Figure 7.

Tethering the flagella triggers an increase in degU transcription.A. Micrographs of cells containing the PdegU–lacZ transcriptional reporter fusion (NRS4351) grown presence or absence of Hag antibody in sera. Micrographs are static images taken from movies filmed 5 min after the addition of antibody to the culture (see Movies S1 and S2). Scale bars represent 100 pixels.B. β-Galactosidase assays of the wild-type strain carrying the PdegU–lacZ transcriptional reporter fusion (NRS4351) grown in the presence of a 1:20 dilution of either pre-immune sera or the Hag-specific antibody. Three independent pre-immune sera were used as controls. Data are plotted as the average of at least three independent replicates. Error bars represent standard error of the mean.