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Mannose cap elongation and mannan core branching disrupted in mptC mutant. Immunoblots of lysates of wildtype M. marinum (WT), the mptC mutant (mptC), the capless mutant (capl), the mptC mutant complemented with M. marinum, M. smegmatis or M. tuberculosis mptC, (mptC + mptC Mm, Msm and Mtb respectively). Samples were separated by SDS-PAGE and probed with (A) Mab F30-5 (α-AraLAM), (B) Mab F55.92.1a1 (α-ManLAM), (C) CV-N-HRP or (D) DC-SIGN-Fc.
